The Pcr Technique

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Principles And Technical Aspects Of Pcr Amplification

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Author : Elizabeth van Pelt-Verkuil
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-03-14

Principles And Technical Aspects Of Pcr Amplification written by Elizabeth van Pelt-Verkuil and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-03-14 with Science categories.

Kary Mullis was awarded a Nobel Prize for inventing the PCR technique more than a decade ago in 1993. Since its "discovery", multiple adaptations and variations of the standard PCR technique have been described. This publication aims to provide the reader with a guide to the standard PCR technique and its many available variants, with particular emphasis being placed on the role of these PCR techniques in the clinical diagnostic laboratory (the central theme of this book).

The Pcr Technique

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Author : James W. Larrick
language : en
Publisher: Eaton Publishing Company/Biotechniques Books
Release Date : 1997

The Pcr Technique written by James W. Larrick and has been published by Eaton Publishing Company/Biotechniques Books this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997 with Medical categories.

A collection of papers originally appearing in BioTechniques, a journal of life science laboratory techniques, from 1991 to 1996, focusing on various aspects of quantitative PCR. Papers present novel methods to quantitate PCR products, improvements and modifications of previously published methods, and applications of quantitative PCR to a variety of clinical situations, especially viral analysis. Most papers are updated by their original authors at the conclusion of the original papers. As of February 1998, Books in Print erroneously shows the publisher to be Biotech Publishing, rather than Eaton Publishing. Annotation copyrighted by Book News, Inc., Portland, OR

Pcr Technology

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Author : Henry Erlich
language : en
Publisher: Springer
Release Date : 2015-12-31

Pcr Technology written by Henry Erlich and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-12-31 with Science categories.

This is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years.

Pcr Protocols

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Author : Michael A. Innis
language : en
Publisher: Academic Press
Release Date : 2012-12-02

Pcr Protocols written by Michael A. Innis and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.

The correct procedures you need for frustration-free PCR methods and applications are contained in this complete, step-by-step, clearly written, inexpensive manual. - Avoid contamination--with specific instructions on setting up your lab - Avoid cumbersome molecular biological techniques - Discover new applications

Pcr Methods And Applications

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Author :
language : en
Publisher:
Release Date : 1995

Pcr Methods And Applications written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995 with Gene amplification categories.

The Pcr Technique

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Author : Ulf Gyllensten
language : en
Publisher: Eaton Publishing Company/Biotechniques Books
Release Date : 1997

The Pcr Technique written by Ulf Gyllensten and has been published by Eaton Publishing Company/Biotechniques Books this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997 with Medical categories.

Applications Of Polymerase Chain Reaction

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Author : Sarika Garg
language : en
Publisher: Arcler Press
Release Date : 2017-11

Applications Of Polymerase Chain Reaction written by Sarika Garg and has been published by Arcler Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-11 with categories.

The polymerase chain reaction (PCR) is a widely used molecular biology technique. It was invented in 1983 by Kary Mullis, who was awarded a Nobel Prize in Chemistry in 1993 for his innovation. Since its introduction, the PCR technology has brought a true revolution in scientific research involving applied areas such as diagnosis and genetic improvements for plants and animals. PCR is a method of replicating deoxyribonucleic acid (DNA) by the selective amplification of a target region of a DNA molecule. Only few cycles of PCR can generate millions of copies of a particular DNA sequence quickly and precisely. Routinely, a PCR reaction consists of three distinct steps- denaturation, annealing and extension. In the first step of denaturation (at 95°C), double stranded DNA denatures into two single strands. In the annealing step (at 56°C), short complementary sequences of DNA known as primers, anneal to the single stranded target DNA. In the final step of extension, temperature is raised to around 72℃. DNA polymerase extends the primer to form a second complimentary strand of DNA. The elaboration of a PCR technique for amplification and for analyzing even very small amounts of DNA or a damaged DNA has generated wide applications in analysis of gene, diagnosis of several genetic diseases and detection of infectious agents like viruses, bacteria, fungi, protozoa, etc. Another beneficial application of PCR is in the generation of a genetic fingerprint. PCR is also used to monitor pathogens in the environment. PCR is an imperative technique for research in molecular biology such as in cloning procedure which has considerable potential in forensic science. PCR has numerous applications in various other fields such as agriculture sciences, personalized medicine, archeology, etc. This book gathers the recently published works related to PCR and its applications. The first chapter highlights the introduction to polymerase chain reaction. The second chapter features the application of reverse transcriptase-polymerase chain reaction (RT-PCR) in the rapid detection of pathogen in water samples. In chapter 3, discussion is on the PCR methods useful for accurate and rapid screening of the genetically modified organisms in foods. PCR applications in forensic science are described in chapter 4. Application of PCR for the diagnosis of ankylosing spondylitis is discussed in chapter 5. In chapters 6-12, use of PCR technique for the detection of pathogens and diagnosis of infectious diseases is described. PCR methods for the detection of Alzheimer's disease-related single nucleotide polymorphism and cancer related genes are illustrated in chapters 13 and 14, respectively. Chapter 15 discusses the PCR in exploring endodontic infections. Analysis of the archaeological skeletal remains using PCR is described in the last chapter of the book. I hope that this book will be advantageous for students, researchers, teachers, and industrial experts.

Pcr Technology

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Author : Thomas Weissensteiner
language : en
Publisher: CRC Press
Release Date : 2003-11-13

Pcr Technology written by Thomas Weissensteiner and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-11-13 with Medical categories.

A technique used to amplify the number of copies of a specific region of DNA, the polymerase chain reaction (PCR) is at the forefront of the dramatic development of biochemistry. This text provides the tools for developing innovative approaches to using this leading technology. It includes theoretical considerations, discussions, and a selection of

Pcr

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Author : Lucília Domingues
language : en
Publisher: Springer Nature
Release Date : 2023-08-22

Pcr written by Lucília Domingues and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-08-22 with Science categories.

This second volume focuses on PCR methods and PCR application specificities to the biotechnology and bioengineering field. New and updated chapters detail real-time PCR protocols, synthetic biology applications, pathogen detection, microfluidics, digital, multiplex detection recent advances. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, PCR: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.

Apoptosis Detection And Assay Methods

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Author : Li Zhu
language : en
Publisher: Eaton Publishing Company/Biotechniques Books
Release Date : 1998

Apoptosis Detection And Assay Methods written by Li Zhu and has been published by Eaton Publishing Company/Biotechniques Books this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998 with Medical categories.