Pcr Methods And Applications
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Pcr Protocols
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Author : Michael A. Innis
language : en
Publisher: Academic Press
Release Date : 2012-12-02
Pcr Protocols written by Michael A. Innis and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.
The correct procedures you need for frustration-free PCR methods and applications are contained in this complete, step-by-step, clearly written, inexpensive manual. - Avoid contamination--with specific instructions on setting up your lab - Avoid cumbersome molecular biological techniques - Discover new applications
Pcr Methods And Applications
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Author :
language : en
Publisher:
Release Date : 1995
Pcr Methods And Applications written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995 with Gene amplification categories.
Pcr Protocols
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Author : Bruce A. White
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Pcr Protocols written by Bruce A. White and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.
PCR has been successfully utilized in every facet of basic, cli- cal, and applied studies of the life sciences, and the impact that PCR has had on life science research is already staggering. C- comitant with the essentially universal use of PCR has been the creative and explosive development of a wide range of PCR-based techniques and applications. These increasingly numerous pro- cols have each had the general effect of facilitating and acceler- ing research. Because PCR technology is relatively easy and inexpensive, PCR applications are well within the reach of every research lab. In this sense, PCR has become the "equalizer" between "small" and "big" labs, since its use makes certain projects, especially those related to molecular cloning, now far more feasible for the small lab with a modest budget. This new volume on PCR Protocols does not attempt the impossible task of representing all PCR-based protocols. Rather, it presents a range of protocols, both analytical and preparative, that provide a solid base of knowledge on the use of PCR in many c- mon research problems. The first six chapters provide some basic information on how to get started. Chapters 7-19 represent primarily analytical uses of PCR, both for simple DNA and RNA detection, as well as for more complex analyses of nucleic acid (e. g. , DNA footprin ting, RNA splice site localization). The remaining chapters represent "synthetic," or preparative, uses of PCR.
Rapid Cycle Real Time Pcr Methods And Applications
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Author : Udo Reischl
language : en
Publisher: Springer
Release Date : 2002
Rapid Cycle Real Time Pcr Methods And Applications written by Udo Reischl and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002 with Medical categories.
Rapid Cycle Real-Time PCR is a powerful analytical tool with broad application for the basic and applied life sciences. Compared with conventional PCR technology, Rapid Cycle Real-Time PCR is faster, has greater specificity, and is more easily adaptable for a variety of diagnostic tests, including qualitative, quantitative and mutation detection assays. This book provides general overviews of this technology for use in the clinical microbiology laboratory as well as specific diagnostic protocols for the detection of viral, bacterial and fungal pathogens and genetically modified organisms in human specimens and foodstuffs. All of these protocols have been developed, verified, and validated by experts in the field and should be of great interest for clinical microbiologists, pathologists, laboratory technologists as well as practicing physicians.
Pcr Technology
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Author : Henry Erlich
language : en
Publisher: Springer
Release Date : 2015-12-31
Pcr Technology written by Henry Erlich and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-12-31 with Science categories.
This is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years.
Pcr
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Author : Lucília Domingues
language : en
Publisher: Springer Nature
Release Date : 2023-08-22
Pcr written by Lucília Domingues and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-08-22 with Science categories.
This second volume focuses on PCR methods and PCR application specificities to the biotechnology and bioengineering field. New and updated chapters detail real-time PCR protocols, synthetic biology applications, pathogen detection, microfluidics, digital, multiplex detection recent advances. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, PCR: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
Rapid Cycle Real Time Pcr Methods And Applications
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Author : Carl Wittwer
language : en
Publisher: Springer
Release Date : 2012-12-06
Rapid Cycle Real Time Pcr Methods And Applications written by Carl Wittwer and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
Rapid cycle real-time PCR is a powerful technique for nucleic acid quantification and analysis that takes less than 30 minutes to complete. Fluorescence is automatically monitored each cycle and the amount of template quantified by advanced analytical methods, such as the second derivative maximum method. Immediately following rapid cycle PCR, melting curve analysis is performed to verify product purity with SYBR Green I and/or genotype with fluorescently-labeled hybridization probes (HybProbes or SimpleProbes). Rapid cycle real-time PCR is often cited as the most versatile, efficient method for nucleic acid quantification in research and climical studies. Molecular analysis has never been easier!
Applications Of Polymerase Chain Reaction
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Author : Sarika Garg
language : en
Publisher: Arcler Press
Release Date : 2017-11
Applications Of Polymerase Chain Reaction written by Sarika Garg and has been published by Arcler Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-11 with categories.
The polymerase chain reaction (PCR) is a widely used molecular biology technique. It was invented in 1983 by Kary Mullis, who was awarded a Nobel Prize in Chemistry in 1993 for his innovation. Since its introduction, the PCR technology has brought a true revolution in scientific research involving applied areas such as diagnosis and genetic improvements for plants and animals. PCR is a method of replicating deoxyribonucleic acid (DNA) by the selective amplification of a target region of a DNA molecule. Only few cycles of PCR can generate millions of copies of a particular DNA sequence quickly and precisely. Routinely, a PCR reaction consists of three distinct steps- denaturation, annealing and extension. In the first step of denaturation (at 95°C), double stranded DNA denatures into two single strands. In the annealing step (at 56°C), short complementary sequences of DNA known as primers, anneal to the single stranded target DNA. In the final step of extension, temperature is raised to around 72℃. DNA polymerase extends the primer to form a second complimentary strand of DNA. The elaboration of a PCR technique for amplification and for analyzing even very small amounts of DNA or a damaged DNA has generated wide applications in analysis of gene, diagnosis of several genetic diseases and detection of infectious agents like viruses, bacteria, fungi, protozoa, etc. Another beneficial application of PCR is in the generation of a genetic fingerprint. PCR is also used to monitor pathogens in the environment. PCR is an imperative technique for research in molecular biology such as in cloning procedure which has considerable potential in forensic science. PCR has numerous applications in various other fields such as agriculture sciences, personalized medicine, archeology, etc. This book gathers the recently published works related to PCR and its applications. The first chapter highlights the introduction to polymerase chain reaction. The second chapter features the application of reverse transcriptase-polymerase chain reaction (RT-PCR) in the rapid detection of pathogen in water samples. In chapter 3, discussion is on the PCR methods useful for accurate and rapid screening of the genetically modified organisms in foods. PCR applications in forensic science are described in chapter 4. Application of PCR for the diagnosis of ankylosing spondylitis is discussed in chapter 5. In chapters 6-12, use of PCR technique for the detection of pathogens and diagnosis of infectious diseases is described. PCR methods for the detection of Alzheimer's disease-related single nucleotide polymorphism and cancer related genes are illustrated in chapters 13 and 14, respectively. Chapter 15 discusses the PCR in exploring endodontic infections. Analysis of the archaeological skeletal remains using PCR is described in the last chapter of the book. I hope that this book will be advantageous for students, researchers, teachers, and industrial experts.
Pcr Protocols
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Author : Michael A. Innis
language : en
Publisher: Academic Press
Release Date : 1990-01-11
Pcr Protocols written by Michael A. Innis and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1990-01-11 with Science categories.
The correct procedures you need for frustration-free PCR methods and applications are contained in this complete, step-by-step, clearly written, inexpensive manual. Avoid contamination--with specific instructions on setting up your lab Avoid cumbersome molecular biological techniques Discover new applications
Pcr Applications
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Author : Michael A. Innis
language : en
Publisher: Academic Press
Release Date : 1999-05-11
Pcr Applications written by Michael A. Innis and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999-05-11 with Science categories.
PCR is the most powerful technique currently used in molecular biology. It enables the scientist to quickly replicate DNA and RNA on the benchtop. From its discovery in the early 80's, PCR has blossomed into a method that enables everything from ready mutation of DNA/RNA to speedy analysis of tens of thousands of nucleotide sequences daily.PCR Applications examines the latest developments in this field. It is the third book in the series, building on the previous publications PCR Protocols and PCR Strategies. The manual discusses techniques that focus on gene discovery, genomics, and DNA array technology, which are contributing factors to the now-occurring bioinformatics boom.Key Features* Focuses on gene discovery, genomics, and DNA array technology* Covers quantitative PCR techniques, including the use of standards and kinetic analysisincludes statistical refinement of primer design parameters* Ilustrates techniques used in microscopic tissue samples, such as single cell PCR, whole cell PCR, laser capture microdissection, and in situ PCREntries provide information on:* Nomenclature* Expression* Sequence analysis* Structure and function* Electrophysiology* Parmacology* Information retrieval