Proteins At Surfaces

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Proteins At Surfaces

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Author : Yulia Mikhailovna Efimova
language : en
Publisher: IOS Press
Release Date : 2006

Proteins At Surfaces written by Yulia Mikhailovna Efimova and has been published by IOS Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006 with Science categories.

Understanding protein adsorption is of vital importance in many fields of medicine and industry that can be divided into two categories: those in which it is desired to minimize adsorption, and those in which protein adsorption is desired. The first category covers materials for kidney dialysis membranes, implants in the bloodstream, vessels for storing pharmaceutical products, marine antifouling paints, etc. The second covers mainly materials for surgical implants, e.g., bone replacement, teeth implants, which must be assimilated with the living tissue. For example, bones or tooth implants require good protein adhesion to allow the growth of the bone and the formation of a stable and strong interphase between the implants and the bone. In contrast, the protein adhesion has to be poor for catheters or contact lenses, where a low contact with the tissue is required...

The Production And Characterization Of Nanostructures Of Single Stranded Dna And Proteins Using A Nanoengineering Approach

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Author : Maozi Liu
language : en
Publisher:
Release Date : 2004

The Production And Characterization Of Nanostructures Of Single Stranded Dna And Proteins Using A Nanoengineering Approach written by Maozi Liu and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with categories.

Wallerstein Laboratories Communications On The Science And Practice Of Brewing

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Author : Wallerstein Laboratories
language : en
Publisher:
Release Date : 1950

Wallerstein Laboratories Communications On The Science And Practice Of Brewing written by Wallerstein Laboratories and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1950 with Beer categories.

Surface And Interfacial Aspects Of Biomedical Polymers Vol 2 Protein Adsorption

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Author : Joseph Andrade
language : en
Publisher: Springer
Release Date : 1985

Surface And Interfacial Aspects Of Biomedical Polymers Vol 2 Protein Adsorption written by Joseph Andrade and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 1985 with Science categories.

Anchoring Of Surface Proteins To The Cell Wall Of Gram Positive Bacteria

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Author : William Wiley Navarre
language : en
Publisher:
Release Date : 1999

Anchoring Of Surface Proteins To The Cell Wall Of Gram Positive Bacteria written by William Wiley Navarre and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999 with categories.

Surface Analysis Of Adsorbed Proteins

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Author : Elaine Hillenmeyer Tronic
language : en
Publisher:
Release Date : 2012

Surface Analysis Of Adsorbed Proteins written by Elaine Hillenmeyer Tronic and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012 with categories.

Adsorbed proteins on surfaces are important in many applications, including medical implants, sensors, marine materials, and in vitro substrates for cell culture and other uses. Understanding the protein structure on the surface would allow better control of the interaction of the material with the surrounding environment and a more reproducible system response. Protein adsorption is a complex process, and characterization requires the combination of multiple analysis techniques. In this thesis, adsorbed protein amounts were measured using x-ray photoelectron spectroscopy (XPS) and radiolabeled proteins. Protein conformation and orientation were measured using time-of-flight secondary ion mass spectrometry (ToF-SIMS), near edge x-ray absorption fine structure (NEXAFS), sum frequency generation (SFG), and enzyme-linked immunosorbent assays (ELISA). Surface type influenced the adsorbed surface concentration of albumin and fibronectin on surfaces of glass, polystyrene, titanium, and sulfonated polystyrene as measured by XPS and radiolabeled protein adsorption. More albumin adsorbed onto polystyrene than glass. More albumin and fibronectin adsorbed onto sulfonated polystyrene than titanium. ToF-SIMS also showed differences in structure of the proteins adsorbed onto the different surfaces. The A1 domain of von Willebrand Factor adsorbed in similar amounts onto glass, tissue culture polystyrene, and polystyrene surfaces, as measured by XPS. However, ToF-SIMS showed differences in solution exposure of A1 domain amino acids when adsorbed onto the three surfaces and NEXAFS showed the most ordered ß-sheet structure when A1 was adsorbed onto polystyrene. ELISA showed lowest binding of antibodies recognizing a nonlinear epitopes within A1 when A1 was adsorbed onto polystyrene. Functional studies using a parallel plate flow chamber measured platelet binding to A1 adsorbed onto the three surfaces. At high shear (20dyne/cm2), platelets showed most detachment from A1 adsorbed onto glass. At low shear (0.2dyne/cm2), platelets showed most detachment from A1 adsorbed onto polystyrene. Surface analysis was also useful in characterizing collagen substrates created under different experimental conditions, including material source and pH. Collagen obtained from different sources exhibited altered adsorption behavior, both in amount adsorbed as measured by XPS and interaction with the A1 domain of von Willebrand Factor as measured by ToF-SIMS. SFG was used to identify differences in ordering of collagen adsorbed from solutions at different pH values. Collagen adsorbed at pH 8.0 showed higher SFG amide signal than collagen adsorbed at pH 6.5, suggesting greater ordering of the peptide backbone at pH 8.0. The differences in SFG signal were not due to the amount adsorbed protein, as XPS showed more collagen adsorbed onto tissue culture polystyrene at pH 6.5 than pH 8.0. These studies demonstrated that the surface type can have a large impact on adsorbed proteins, both in amount adsorbed and surface structure. These studies also showed that surface analysis is very useful in creating defined in vitro protein substrates. In all cases, it was crucial to use multiple analysis techniques to understand these systems.

Proteins At Interfaces

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Author : John L. Brash
language : en
Publisher:
Release Date : 1987

Proteins At Interfaces written by John L. Brash and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1987 with Science categories.

Gastrulation

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Author : Claudio D. Stern
language : en
Publisher: CSHL Press
Release Date : 2004

Gastrulation written by Claudio D. Stern and has been published by CSHL Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Science categories.

During gastrulation, tissue layers are formed and the overall body plan is established. This book is the definitive guide to this vitally important period in embryonic development, providing authoritative and up to date information that includes the first comprehensive interspecies comparison, cell movements and patterning events, the roles of individual genes and gene families, and the evolution of gastrulation.

Heat Shock Proteins And Gamma Delta T Cells

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Author : Celia F. Brosnan
language : en
Publisher: S. Karger AG (Switzerland)
Release Date : 1992

Heat Shock Proteins And Gamma Delta T Cells written by Celia F. Brosnan and has been published by S. Karger AG (Switzerland) this book supported file pdf, txt, epub, kindle and other format this book has been release on 1992 with Medical categories.

Biological Performance Of Materials

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Author : Jonathan Black
language : en
Publisher: Marcel Dekker
Release Date : 1992

Biological Performance Of Materials written by Jonathan Black and has been published by Marcel Dekker this book supported file pdf, txt, epub, kindle and other format this book has been release on 1992 with Medical categories.