Protein Engineering Techniques
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Protein Engineering Techniques
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Author : Krishna Mohan Poluri
language : en
Publisher: Springer
Release Date : 2016-11-12
Protein Engineering Techniques written by Krishna Mohan Poluri and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-11-12 with Technology & Engineering categories.
This brief provides a broad overview of protein-engineering research, offering a glimpse of the most common experimental methods. It also presents various computational programs with applications that are widely used in directed evolution, computational and de novo protein design. Further, it sheds light on the advantages and pitfalls of existing methodologies and future perspectives of protein engineering techniques.
Enzymes For Biotechnology
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Author :
language : en
Publisher:
Release Date : 1988
Enzymes For Biotechnology written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1988 with categories.
Protein Engineering Protocols
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Author : Kristian Müller
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-10-26
Protein Engineering Protocols written by Kristian Müller and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-10-26 with Science categories.
Protein engineering is a fascinating mixture of molecular biology, protein structure analysis, computation, and biochemistry, with the goal of developing useful or valuable proteins. Protein Engineering Protocols will consider the two general, but not mutually exclusive, strategies for protein engineering. The first is known as rational design, in which the scientist uses detailed knowledge of the structure and function of the protein to make desired changes. The s- ond strategy is known as directed evolution. In this case, random mutagenesis is applied to a protein, and selection or screening is used to pick out variants that have the desired qualities. By several rounds of mutation and selection, this method mimics natural evolution. An additional technique known as DNA shuffling mixes and matches pieces of successful variants to produce better results. This process mimics recombination that occurs naturally during sexual reproduction. The first section of Protein Engineering Protocols describes rational p- tein design strategies, including computational methods, the use of non-natural amino acids to expand the biological alphabet, as well as impressive examples for the generation of proteins with novel characteristics. Although procedures for the introduction of mutations have become routine, predicting and und- standing the effects of these mutations can be very challenging and requires profound knowledge of the system as well as protein structures in general.
A Practical Guide To Protein Engineering
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Author : Tuck Seng Wong
language : en
Publisher: Springer Nature
Release Date : 2020-10-29
A Practical Guide To Protein Engineering written by Tuck Seng Wong and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-10-29 with Science categories.
This textbook introduces readers in an accessible and engaging way to the nuts and bolts of protein expression and engineering. Various case studies illustrate each step from the early sequence searches in online databases over plasmid design and molecular cloning techniques to protein purification and characterization. Furthermore, readers are provided with practical tips to successfully pursue a career as a protein engineer. With protein engineering being a fundamental technique in almost all molecular biology labs, the book targets advanced undergraduates and graduate students working in molecular biology, biotechnology and related scientific fields.
Dna And Protein Engineering Techniques
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Author : Dpet
language : en
Publisher:
Release Date : 1990-01-01
Dna And Protein Engineering Techniques written by Dpet and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1990-01-01 with categories.
Protein Engineering And Design
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Author : Paul R. Carey
language : en
Publisher: Elsevier
Release Date : 1996-06-21
Protein Engineering And Design written by Paul R. Carey and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996-06-21 with Science categories.
The design and production of novel peptides and proteins occupy pivotal positions in science and technology and will continue to do so in the 21st century. Protein Engineering and Design outlines the rapid advances in computer-based modeling, protein engineering, and methods needed for protein and peptide preparation and characterization. This indispensable reference lays the groundwork for understanding this multidisciplinary activity while providing an introduction for researchers and students to the field of protein design. - Introduces and defines the techniques involved in protein engineering and design - Provides a concise overview of key technologies involved and demonstrates their contributions to the specialized design and production of novel proteins and peptides
Protein Structure And Protein Engineering
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Author : Ernst-Ludwig Winnacker
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Protein Structure And Protein Engineering written by Ernst-Ludwig Winnacker and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
Protein engineering has had considerable impact on basic and applied research in biochemistry and molecular biology. It is already in use as a tool in molecular biology, but it is beginning to strongly influence the planning of experiments in biology everywhere, and, with even fur ther-reaching consequences, the appointment politics in research in stitutions and industries. Protein engineering, perhaps more than any other methods of protein analysis and peptide synthesis, has shown that proteins are organic molecules governed by the universal laws of chemistry and physics. How ever, as was the case with other new powerful methods and techniques, protein engineering tempts to an exploration of its limitations and thus generates more questions than it answers. The 39th Mosbacher Colloquium on Protein Structure and Protein Engi neering is not the first conference on this topic and it will not be the last. The important issues are obviously techniques of protein en gineering, examples of application, and the basic framework of protein structure and stability within which reasonable experiments can be de signed; conversely also, what we can learn about protein structure, dynamics, and folding from such experiments. Experiments in this direc tion aim at elucidating the folding code in the long run, but help to exploit the role of individual amino acid residues in catalysis, pro tein stability, and binding specificity in selected proteins now.
Biotechnology For Beginners
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Author : Reinhard Renneberg
language : en
Publisher: Elsevier
Release Date : 2008
Biotechnology For Beginners written by Reinhard Renneberg and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008 with Science categories.
Offering an exciting and colorful overview of biotechnology for professionals and students in a wide array of the life sciences, this book also appeals to the lay reader without a scientific background who is interested in an entertaining and informative introduction to the key aspects of biotechnology.
Molecular Biology Of Protein Folding Part B
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Author : P. Michael Conn
language : en
Publisher: Academic Press
Release Date : 2009-01-09
Molecular Biology Of Protein Folding Part B written by P. Michael Conn and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-01-09 with Science categories.
Nucleic acids are the fundamental building blocks of DNA and RNA and are found in virtually every living cell. Molecular biology is a branch of science that studies the physicochemical properties of molecules in a cell, including nucleic acids, proteins, and enzymes. Increased understanding of nucleic acids and their role in molecular biology will further many of the biological sciences including genetics, biochemistry, and cell biology. Progress in Nucleic Acid Research and Molecular Biology is intended to bring to light the most recent advances in these overlapping disciplines with a timely compilation of reviews comprising each volume. Follow the new editor-in-chief, P. Michael Conn, as he introduces this second thematic volume in the series – an in-depth aid to researchers who are looking for the best techniques and tools for understanding the complexities of protein folding Understand the advantages of protein folding over other therapeutic approaches and see how protein folding plays a critical role in the development of diseases such as Alzheimer’s and diabetes Decipher the rules of protein folding through compelling and timely reviews combined with chapters written by international authors in engineering, biochemistry, physics and computer science
Protein Engineering
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Author : Tomohisa Ogawa
language : en
Publisher: BoD – Books on Demand
Release Date : 2013-05-29
Protein Engineering written by Tomohisa Ogawa and has been published by BoD – Books on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-05-29 with Science categories.
Given the centrality of protein to many biological process, this book makes a significant contribution to the fields of healthcare and nutrition. Its chapters consider topics such as protein-protein and protein-ligand docking, and the protein engineering of enzymes involved in bioplastic metabolism. One contribution gives an overview of the In Vitro Virus (IVV) analytic method, while another shows how cutting-edge techniques in protein engineering advance our knowledge in the field of palaeontology. The book also includes a review of classic and alternative strategies when using yeasts in research, with a focus on Pichia pastoris as a host. Finally, there are two contributions on chromatography: one on the method itself, and another on its use to identify HMGB1-binding components.