Protein Engineering And Design

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Protein Engineering And Design

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Author : Paul R. Carey
language : en
Publisher: Elsevier
Release Date : 1996-06-21

Protein Engineering And Design written by Paul R. Carey and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996-06-21 with Science categories.

The design and production of novel peptides and proteins occupy pivotal positions in science and technology and will continue to do so in the 21st century. Protein Engineering and Design outlines the rapid advances in computer-based modeling, protein engineering, and methods needed for protein and peptide preparation and characterization. This indispensable reference lays the groundwork for understanding this multidisciplinary activity while providing an introduction for researchers and students to the field of protein design. - Introduces and defines the techniques involved in protein engineering and design - Provides a concise overview of key technologies involved and demonstrates their contributions to the specialized design and production of novel proteins and peptides

Protein Engineering

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Author : Mallorie N. Sheehan
language : en
Publisher:
Release Date : 2013

Protein Engineering written by Mallorie N. Sheehan and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013 with Protein engineering categories.

Concepts In Protein Engineering And Design

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Author : Paul Wrede
language : en
Publisher: Walter de Gruyter GmbH & Co KG
Release Date : 2020-10-12

Concepts In Protein Engineering And Design written by Paul Wrede and has been published by Walter de Gruyter GmbH & Co KG this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-10-12 with Science categories.

No detailed description available for "Concepts in Protein Engineering and Design".

Protein Engineering

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Author :
language : en
Publisher:
Release Date : 1994

Protein Engineering written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994 with categories.

Protein Engineering

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Author : Morio Ikehara
language : en
Publisher: Springer
Release Date : 1990

Protein Engineering written by Morio Ikehara and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 1990 with Science categories.

Methods In Protein Design

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Author :
language : en
Publisher: Academic Press
Release Date : 2013-02-14

Methods In Protein Design written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-02-14 with Science categories.

This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers methods in protein design and it has chapters on such topics as protein switch engineering by domain insertion, evolution based design of proteins, and computationally designed proteins. - Continues the legacy of this premier serial with quality chapters authored by leaders in the field - Covers methods in protein design - Contains chapters with such topics as protein switch engineering by domain insertion, evolution-based design of proteins, and computationally designed proteins

Protein Engineering And Design With An Expanded Amino Acid Repertoire

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Author : Nediljko Budisa
language : en
Publisher:
Release Date : 2005

Protein Engineering And Design With An Expanded Amino Acid Repertoire written by Nediljko Budisa and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005 with categories.

Electrostatics In Protein Engineering And Design

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Author : I. John Khan
language : en
Publisher:
Release Date : 2012

Electrostatics In Protein Engineering And Design written by I. John Khan and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012 with Technology categories.

Electrostatics in Protein Engineering and Design.

Protein Engineering Protocols

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Author : Kristian Müller
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-10-26

Protein Engineering Protocols written by Kristian Müller and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-10-26 with Science categories.

Protein engineering is a fascinating mixture of molecular biology, protein structure analysis, computation, and biochemistry, with the goal of developing useful or valuable proteins. Protein Engineering Protocols will consider the two general, but not mutually exclusive, strategies for protein engineering. The first is known as rational design, in which the scientist uses detailed knowledge of the structure and function of the protein to make desired changes. The s- ond strategy is known as directed evolution. In this case, random mutagenesis is applied to a protein, and selection or screening is used to pick out variants that have the desired qualities. By several rounds of mutation and selection, this method mimics natural evolution. An additional technique known as DNA shuffling mixes and matches pieces of successful variants to produce better results. This process mimics recombination that occurs naturally during sexual reproduction. The first section of Protein Engineering Protocols describes rational p- tein design strategies, including computational methods, the use of non-natural amino acids to expand the biological alphabet, as well as impressive examples for the generation of proteins with novel characteristics. Although procedures for the introduction of mutations have become routine, predicting and und- standing the effects of these mutations can be very challenging and requires profound knowledge of the system as well as protein structures in general.

Protein Engineering And Design Of Thermostable Biosensors

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Author : Yaji Tian
language : en
Publisher:
Release Date : 2007

Protein Engineering And Design Of Thermostable Biosensors written by Yaji Tian and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007 with Protein engineering categories.

In addition, E. coli ribose-binding protein (ecRBP) has been stabilized by rational protein engineering to enhance its suitability as a scaffold protein for use in computational design. Several approaches have been exploited to improve the thermostability of ecRBP, including the introduction of mutations to decrease the entropy of the unfolded form, the replacement of un-favored polar amino-acids in the protein core with non-polar residues, the engineering of disulfide bonds, and the incorporation of features from thermophilic RBPs. The stabilizing mutations achieved from these approaches were evaluated individually and then combined in a stepwise manner, resulting in a variant with a melting temperature 17.5°C higher than ecRBP, which can also serve as a stable scaffold protein for biosensor design.