Pcr Applications
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Pcr Protocols
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Author : Michael A. Innis
language : en
Publisher: Academic Press
Release Date : 2012-12-02
Pcr Protocols written by Michael A. Innis and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.
The correct procedures you need for frustration-free PCR methods and applications are contained in this complete, step-by-step, clearly written, inexpensive manual. - Avoid contamination--with specific instructions on setting up your lab - Avoid cumbersome molecular biological techniques - Discover new applications
Pcr Protocols
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Author : Bruce A. White
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Pcr Protocols written by Bruce A. White and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.
PCR has been successfully utilized in every facet of basic, cli- cal, and applied studies of the life sciences, and the impact that PCR has had on life science research is already staggering. C- comitant with the essentially universal use of PCR has been the creative and explosive development of a wide range of PCR-based techniques and applications. These increasingly numerous pro- cols have each had the general effect of facilitating and acceler- ing research. Because PCR technology is relatively easy and inexpensive, PCR applications are well within the reach of every research lab. In this sense, PCR has become the "equalizer" between "small" and "big" labs, since its use makes certain projects, especially those related to molecular cloning, now far more feasible for the small lab with a modest budget. This new volume on PCR Protocols does not attempt the impossible task of representing all PCR-based protocols. Rather, it presents a range of protocols, both analytical and preparative, that provide a solid base of knowledge on the use of PCR in many c- mon research problems. The first six chapters provide some basic information on how to get started. Chapters 7-19 represent primarily analytical uses of PCR, both for simple DNA and RNA detection, as well as for more complex analyses of nucleic acid (e. g. , DNA footprin ting, RNA splice site localization). The remaining chapters represent "synthetic," or preparative, uses of PCR.
Pcr Technology
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Author : Henry Erlich
language : en
Publisher: Springer
Release Date : 2015-12-31
Pcr Technology written by Henry Erlich and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-12-31 with Science categories.
This is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years.
Pcr Methods And Applications
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Author :
language : en
Publisher:
Release Date : 1995
Pcr Methods And Applications written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995 with Gene amplification categories.
Pcr Applications In Pathology
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Author : David S. Latchman
language : en
Publisher:
Release Date : 1995
Pcr Applications In Pathology written by David S. Latchman and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995 with Medical categories.
The polymerase chain reaction has revolutionized molecular biology by allowing the amplification and characterization of minute amounts of nucleic acids. As well as being of use to basic scientists, this technique is of immense importance to pathologists who routinely need to detect and characterize small amounts of infectious micro-organisms, or to identify mutations within small amounts of human DNA. This essential handbook outlines clearly and concisely the applications of PCR in the area of infectious and inherited diseases so that methods can be readily used by non-molecular biologists who need them.
Applications Of Polymerase Chain Reaction
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Author : Sarika Garg
language : en
Publisher: Arcler Press
Release Date : 2017-11
Applications Of Polymerase Chain Reaction written by Sarika Garg and has been published by Arcler Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-11 with categories.
The polymerase chain reaction (PCR) is a widely used molecular biology technique. It was invented in 1983 by Kary Mullis, who was awarded a Nobel Prize in Chemistry in 1993 for his innovation. Since its introduction, the PCR technology has brought a true revolution in scientific research involving applied areas such as diagnosis and genetic improvements for plants and animals. PCR is a method of replicating deoxyribonucleic acid (DNA) by the selective amplification of a target region of a DNA molecule. Only few cycles of PCR can generate millions of copies of a particular DNA sequence quickly and precisely. Routinely, a PCR reaction consists of three distinct steps- denaturation, annealing and extension. In the first step of denaturation (at 95°C), double stranded DNA denatures into two single strands. In the annealing step (at 56°C), short complementary sequences of DNA known as primers, anneal to the single stranded target DNA. In the final step of extension, temperature is raised to around 72℃. DNA polymerase extends the primer to form a second complimentary strand of DNA. The elaboration of a PCR technique for amplification and for analyzing even very small amounts of DNA or a damaged DNA has generated wide applications in analysis of gene, diagnosis of several genetic diseases and detection of infectious agents like viruses, bacteria, fungi, protozoa, etc. Another beneficial application of PCR is in the generation of a genetic fingerprint. PCR is also used to monitor pathogens in the environment. PCR is an imperative technique for research in molecular biology such as in cloning procedure which has considerable potential in forensic science. PCR has numerous applications in various other fields such as agriculture sciences, personalized medicine, archeology, etc. This book gathers the recently published works related to PCR and its applications. The first chapter highlights the introduction to polymerase chain reaction. The second chapter features the application of reverse transcriptase-polymerase chain reaction (RT-PCR) in the rapid detection of pathogen in water samples. In chapter 3, discussion is on the PCR methods useful for accurate and rapid screening of the genetically modified organisms in foods. PCR applications in forensic science are described in chapter 4. Application of PCR for the diagnosis of ankylosing spondylitis is discussed in chapter 5. In chapters 6-12, use of PCR technique for the detection of pathogens and diagnosis of infectious diseases is described. PCR methods for the detection of Alzheimer's disease-related single nucleotide polymorphism and cancer related genes are illustrated in chapters 13 and 14, respectively. Chapter 15 discusses the PCR in exploring endodontic infections. Analysis of the archaeological skeletal remains using PCR is described in the last chapter of the book. I hope that this book will be advantageous for students, researchers, teachers, and industrial experts.
Pcr Applications
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Author : Michael A. Innis
language : en
Publisher: Academic Press
Release Date : 1999-05-11
Pcr Applications written by Michael A. Innis and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999-05-11 with Science categories.
PCR is the most powerful technique currently used in molecular biology. It enables the scientist to quickly replicate DNA and RNA on the benchtop. From its discovery in the early 80's, PCR has blossomed into a method that enables everything from ready mutation of DNA/RNA to speedy analysis of tens of thousands of nucleotide sequences daily.PCR Applications examines the latest developments in this field. It is the third book in the series, building on the previous publications PCR Protocols and PCR Strategies. The manual discusses techniques that focus on gene discovery, genomics, and DNA array technology, which are contributing factors to the now-occurring bioinformatics boom.Key Features* Focuses on gene discovery, genomics, and DNA array technology* Covers quantitative PCR techniques, including the use of standards and kinetic analysisincludes statistical refinement of primer design parameters* Ilustrates techniques used in microscopic tissue samples, such as single cell PCR, whole cell PCR, laser capture microdissection, and in situ PCREntries provide information on:* Nomenclature* Expression* Sequence analysis* Structure and function* Electrophysiology* Parmacology* Information retrieval
Applications Of Pcr In Mycology
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Author : P. D. Bridge
language : en
Publisher: CABI
Release Date : 1998-01-01
Applications Of Pcr In Mycology written by P. D. Bridge and has been published by CABI this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998-01-01 with Science categories.
Since the initial report of the amplification of specific DNA fragments using the polymerase chain reaction (PCR) in 1985, this technique has revolutionized molecular biology. It enables the production of large quantities of DNA from minute amounts of sample material, which can then be readily analyzed. This facility has had an enormous influence on the way both fundamental and diagnostic questions are approached and its use is now considered essential for molecular work in all branches of biology. The purpose of this book is to highlight the wide-ranging applications of PCR in pure and applied mycology and to increase understanding of its potential benefits. After a brief overview, a group of internationally-renowned mycologists give definitive descriptions of the use of PCR in their own specialized fields. These include fungal gene expression and cloning, taxonomy and speciation, fungal mycobionts, mycorrhizal fungi, entomopathogenic fungi, mycotoxin-producing fungi, diagnosis of fungal infections in animals, seed-borne diseases, fungal/plant interactions and applications with industrially-important fungi. Finally, potential future directions for PCR work in mycology are discussed.
Pcr Technology
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Author : Henry A. Erlich
language : en
Publisher: Oxford University Press, USA
Release Date : 1992
Pcr Technology written by Henry A. Erlich and has been published by Oxford University Press, USA this book supported file pdf, txt, epub, kindle and other format this book has been release on 1992 with DNA categories.
Polymerase chain reaction (PCR) technology is a revolutionary innovation which enables scientists to rapidly generate large amounts of genetic material from a slight trace which would otherwise be too small to analyze. With applications in both research and diagnostics, PCR is becoming a standard procedure in biotechnology and medical diagnostic laboratories. This book is an introduction and guide to the new technology, covering the basic methodologies and their applications in research and medicine, emphasizing practical aspects. Each chapter is written by pioneers in the field and most include detailed protocols and favorite PCR "recipes". Students and researchers in all areas of biotechnology and molecular biology will find this book the introduction to PCR they've been looking for.
Clinical Applications Of Pcr
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Author : Y. M. Dennis Lo
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-01
Clinical Applications Of Pcr written by Y. M. Dennis Lo and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-01 with Science categories.
The polymerase chain reaction (PCR) is one of the most important molecular biological methods ever devised, with numerous applications to cli- cal molecular medicine. Since its description in 1985, PCR has undergone tremendous improvements, and many variations on the basic PCR theme have been published. With such a large volume of PCR-related literature, a clinical scientist wishing to use the technique will have a difficult task loc- ing the relevant information to implement it effectively. There is thus clearly a need for an up-to-date volume with detailed protocols to facilitate the setting up of those techniques most relevant to clinical applications. Unlike some other books on this topic, Clinical Applications of PCR includes only methods that are of direct relevance in clinical settings. The book is organized in three parts: an introductory section, a section on general methodology, and a final section with specific clinical applications. The first section covers the basic principles of PCR and is most useful to those new to molecular diagnosis. The next chapter includes useful tips for setting up a PCR laboratory. Section 2 then outlines some of the most commonly used PCR-based techniques in molecular diagnosis. Section 3 includes carefully chosen examples that represent typical applications of PCR in diverse clinical fields, encompassing hematology, oncology, genetics, and microbiology.