The Yeast Two Hybrid System
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The Yeast Two Hybrid System
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Author : Paul L. Bartel
language : en
Publisher:
Release Date : 1997
The Yeast Two Hybrid System written by Paul L. Bartel and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997 with Science categories.
Biologists have already identified thousands of new gene sequences, and genome sequencing efforts are speeding up the discovery process even further. With this explosion of sequence information comes the need to understand how genes work in concert in order to fulfill the cells functions. The yeast two-hybrid system--used to identify protein-protein interactions--is one of the most powerful and versatile methods for characterizing a protein's function. It has become an essential tool for both academic researchers and those in biotechnology and pharmaceutical companies. This volume presents work by pioneers in the field and is the first publication devoted solely to the yeast two-hybrid system. It includes detailed protocols, practical advice on troubleshooting, and suggestions for future development. In addition, it explains how to construct an activation domain hybrid library, how to identify mutations that disrupt an interaction, and how to use the system in mammalian cells. Many of the contributors have developed new applications and variations of the technique. Chapter topics include characterizing hormone/receptor complexes, identifying peptide ligands, analyzing interactions mediated by protein modifications, and dissecting the cell cycle and other complex genetic networks. The Yeast Two-Hybrid System is the single complete resource for scientists interested in this powerful research method.
Two Hybrid Systems
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Author : Paul N. MacDonald
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Two Hybrid Systems written by Paul N. MacDonald and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
Paul N. MacDonald has assembled a collection of powerful molecular tools for examining and characterizing protein-protein, protein-DNA, and protein-RNA interactions. The techniques range from the most basic (introducing plasmids into yeasts, interaction assays, and recovering the plasmids from yeast), to the most advanced alternative strategies (involving one-hybrid, split two-hybrid, three-hybrid, membrane recruitment systems, and mammalian systems). Methods are also provided for dealing with the well-known problem of artifacts and false positives and for identifying the interacting partners in important biological systems, including the SMAD and nuclear receptor pathways. To ensure ready reproducibility and robust results, each technique is described in step-by-step detail by researchers who employ it regularly.
Finding Interactions Of Scrambled By Using The Yeast Two Hybrid System
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Author : Jacqueline Phuong Anh Nguyen
language : en
Publisher:
Release Date : 2008
Finding Interactions Of Scrambled By Using The Yeast Two Hybrid System written by Jacqueline Phuong Anh Nguyen and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008 with categories.
Many crop plants, like soybean and canola, have pods that open to release seeds. However, the molecular mechanism of this pod-opening process is not well understood. I have studied two genes, called SCRAMBLED (SCM) and QUIRKY (QKY), that regulate this process in Arabidopsis thaliana. SCM encodes for a leucine-rich repeat receptor-like kinase and QKY encodes for a transmembrane protein with C2 domains. Mutations in these genes cause the plants to have small, twisted, and non-shattering pods contrasting to wild type pods, which are long, straight, and shattering. I carried out a double mutant analysis to determine if SCM and QKY genes function in the same genetic pathway. scm qky double mutants show very similar phenotypes to scm and qky phenotypes indicating that both genes function in the same genetic pathway. Using yeast two-hybrid assay, I showed that SCM and QKY proteins do not bind to each other in yeast. In addition, I screened a flower cDNA library using the yeast two-hybrid system for interactors of QKY, SCM extracellular domain, and SCM kinase domain. I found many interactors of SCM in yeast. However, so far, knock-out mutants of the genes did not show scm-like phenotypes. Further tests need to be done in order to determine if any of these putative interactors of SCM actually bind to SCM in Arabidopsis.
Interaction Of The Dna Repair Proteins In The Yeast Two Hybrid System
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Author : Debra L. Parchaliuk
language : en
Publisher:
Release Date : 1996
Interaction Of The Dna Repair Proteins In The Yeast Two Hybrid System written by Debra L. Parchaliuk and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996 with DNA ligases categories.
Understanding The Mechanism Of Agrobacterium Tumefaciens Transformation
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Author : Cheryl Anne Hackworth
language : en
Publisher:
Release Date : 2006
Understanding The Mechanism Of Agrobacterium Tumefaciens Transformation written by Cheryl Anne Hackworth and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006 with categories.
The Use Of The Yeast Two Hybrid System As A Means Of Identifying Protein Interactors Of The Human Protein Bmi 1
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Author : Robert George Edward Holgate
language : en
Publisher:
Release Date : 1999
The Use Of The Yeast Two Hybrid System As A Means Of Identifying Protein Interactors Of The Human Protein Bmi 1 written by Robert George Edward Holgate and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999 with categories.
Investigations Of Alternative Dimerization Partners For The Arylhydrocarbon Receptor
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Author : Ryan Lane Bradley
language : en
Publisher:
Release Date : 1997
Investigations Of Alternative Dimerization Partners For The Arylhydrocarbon Receptor written by Ryan Lane Bradley and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997 with categories.
Proceedings Of The National Academy Of Sciences Of The United States Of America
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Author : National Academy of Sciences (U.S.)
language : en
Publisher:
Release Date : 2005
Proceedings Of The National Academy Of Sciences Of The United States Of America written by National Academy of Sciences (U.S.) and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005 with Science categories.
Science
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Author : John Michels (Journalist)
language : en
Publisher:
Release Date : 2004
Science written by John Michels (Journalist) and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Science categories.
A weekly record of scientific progress.
The Journal Of Cell Biology
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Author :
language : en
Publisher:
Release Date : 2007
The Journal Of Cell Biology written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007 with Biochemistry categories.
No. 2, pt. 2 of November issue each year from v. 19 (1963)-47 (1970) and v. 55 (1972)- contain the Abstracts of papers presented at the Annual Meeting of the American Society for Cell Biology, 3d (1963)-10th (1970) and 12th (1972)-