Single Cell Omics
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Single Cell Omics
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Author : Debmalya Barh
language : en
Publisher: Academic Press
Release Date : 2019-07-30
Single Cell Omics written by Debmalya Barh and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-07-30 with Medical categories.
Single-cell Omics, Volume 2: Advances in Applications provides the latest single-cell omics applications in the field of biomedicine. The advent of omics technologies have enabled us to identify the differences between cell types and subpopulations at the level of the genome, proteome, transcriptome, epigenome, and in several other fields of omics. The book is divided into two sections: the first is dedicated to biomedical applications, such as cell diagnostics, non-invasive prenatal testing (NIPT), circulating tumor cells, breast cancer, gliomas, nervous systems and autoimmune disorders, and more. The second focuses on cell omics in plants, discussing micro algal and single cell omics, and more. This book is a valuable source for bioinformaticians, molecular diagnostic researchers, clinicians and several members of biomedical field interested in understanding more about single-cell omics and its potential for research and diagnosis. - Covers the diverse single cell omics applications in the biomedical field - Summarizes the latest progress in single cell omics and discusses potential future developments for research and diagnosis - Written by experts across the world, it brings different points-of-view and study cases to fully give a comprehensive overview of the topic
Introduction To Single Cell Omics
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Author : Xinghua Pan
language : en
Publisher: Frontiers Media SA
Release Date : 2019-09-19
Introduction To Single Cell Omics written by Xinghua Pan and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-09-19 with categories.
Single-cell omics is a progressing frontier that stems from the sequencing of the human genome and the development of omics technologies, particularly genomics, transcriptomics, epigenomics and proteomics, but the sensitivity is now improved to single-cell level. The new generation of methodologies, especially the next generation sequencing (NGS) technology, plays a leading role in genomics related fields; however, the conventional techniques of omics require number of cells to be large, usually on the order of millions of cells, which is hardly accessible in some cases. More importantly, harnessing the power of omics technologies and applying those at the single-cell level are crucial since every cell is specific and unique, and almost every cell population in every systems, derived in either vivo or in vitro, is heterogeneous. Deciphering the heterogeneity of the cell population hence becomes critical for recognizing the mechanism and significance of the system. However, without an extensive examination of individual cells, a massive analysis of cell population would only give an average output of the cells, but neglect the differences among cells. Single-cell omics seeks to study a number of individual cells in parallel for their different dimensions of molecular profile on genome-wide scale, providing unprecedented resolution for the interpretation of both the structure and function of an organ, tissue or other system, as well as the interaction (and communication) and dynamics of single cells or subpopulations of cells and their lineages. Importantly single-cell omics enables the identification of a minor subpopulation of cells that may play a critical role in biological process over a dominant subpolulation such as a cancer and a developing organ. It provides an ultra-sensitive tool for us to clarify specific molecular mechanisms and pathways and reveal the nature of cell heterogeneity. Besides, it also empowers the clinical investigation of patients when facing a very low quantity of cell available for analysis, such as noninvasive cancer screening with circulating tumor cells (CTC), noninvasive prenatal diagnostics (NIPD) and preimplantation genetic test (PGT) for in vitro fertilization. Single-cell omics greatly promotes the understanding of life at a more fundamental level, bring vast applications in medicine. Accordingly, single-cell omics is also called as single-cell analysis or single-cell biology. Within only a couple of years, single-cell omics, especially transcriptomic sequencing (scRNA-seq), whole genome and exome sequencing (scWGS, scWES), has become robust and broadly accessible. Besides the existing technologies, recently, multiplexing barcode design and combinatorial indexing technology, in combination with microfluidic platform exampled by Drop-seq, or even being independent of microfluidic platform but using a regular PCR-plate, enable us a greater capacity of single cell analysis, switching from one single cell to thousands of single cells in a single test. The unique molecular identifiers (UMIs) allow the amplification bias among the original molecules to be corrected faithfully, resulting in a reliable quantitative measurement of omics in single cells. Of late, a variety of single-cell epigenomics analyses are becoming sophisticated, particularly single cell chromatin accessibility (scATAC-seq) and CpG methylation profiling (scBS-seq, scRRBS-seq). High resolution single molecular Fluorescence in situ hybridization (smFISH) and its revolutionary versions (ex. seqFISH, MERFISH, and so on), in addition to the spatial transcriptome sequencing, make the native relationship of the individual cells of a tissue to be in 3D or 4D format visually and quantitatively clarified. On the other hand, CRISPR/cas9 editing-based In vivo lineage tracing methods enable dynamic profile of a whole developmental process to be accurately displayed. Multi-omics analysis facilitates the study of multi-dimensional regulation and relationship of different elements of the central dogma in a single cell, as well as permitting a clear dissection of the complicated omics heterogeneity of a system. Last but not the least, the technology, biological noise, sequence dropout, and batch effect bring a huge challenge to the bioinformatics of single cell omics. While significant progress in the data analysis has been made since then, revolutionary theory and algorithm logics for single cell omics are expected. Indeed, single-cell analysis exert considerable impacts on the fields of biological studies, particularly cancers, neuron and neural system, stem cells, embryo development and immune system; other than that, it also tremendously motivates pharmaceutic RD, clinical diagnosis and monitoring, as well as precision medicine. This book hereby summarizes the recent developments and general considerations of single-cell analysis, with a detailed presentation on selected technologies and applications. Starting with the experimental design on single-cell omics, the book then emphasizes the consideration on heterogeneity of cancer and other systems. It also gives an introduction of the basic methods and key facts for bioinformatics analysis. Secondary, this book provides a summary of two types of popular technologies, the fundamental tools on single-cell isolation, and the developments of single cell multi-omics, followed by descriptions of FISH technologies, though other popular technologies are not covered here due to the fact that they are intensively described here and there recently. Finally, the book illustrates an elastomer-based integrated fluidic circuit that allows a connection between single cell functional studies combining stimulation, response, imaging and measurement, and corresponding single cell sequencing. This is a model system for single cell functional genomics. In addition, it reports a pipeline for single-cell proteomics with an analysis of the early development of Xenopus embryo, a single-cell qRT-PCR application that defined the subpopulations related to cell cycling, and a new method for synergistic assembly of single cell genome with sequencing of amplification product by phi29 DNA polymerase. Due to the tremendous progresses of single-cell omics in recent years, the topics covered here are incomplete, but each individual topic is excellently addressed, significantly interesting and beneficial to scientists working in or affiliated with this field.
Single Cell Omics
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Author : Debmalya Barh
language : en
Publisher: Academic Press
Release Date : 2019-06-06
Single Cell Omics written by Debmalya Barh and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-06-06 with Medical categories.
Single-Cell Omics: Volume 1: Technological Advances and Applications provides the latest technological developments and applications of single-cell technologies in the field of biomedicine. In the current era of precision medicine, the single-cell omics technology is highly promising due to its potential in diagnosis, prognosis and therapeutics. Sections in the book cover single-cell omics research and applications, diverse technologies applied in the topic, such as pangenomics, metabolomics, and multi-omics of single cells, data analysis, and several applications of single-cell omics within the biomedical field, for example in cancer, metabolic and neuro diseases, immunology, pharmacogenomics, personalized medicine and reproductive health. This book is a valuable source for bioinformaticians, molecular diagnostic researchers, clinicians and members of the biomedical field who are interested in understanding more about single-cell omics and its potential for research and diagnosis. - Covers not only the technological aspects, but also the diverse applications of single cell omics in the biomedical field - Summarizes the latest progress in single cell omics and discusses potential future developments for research and diagnosis - Written by experts across the world, bringing different points-of-view and case studies to give a comprehensive overview on the topic
Understanding Single Cell Omics
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Author : Hebooks
language : en
Publisher: Hebooks
Release Date :
Understanding Single Cell Omics written by Hebooks and has been published by Hebooks this book supported file pdf, txt, epub, kindle and other format this book has been release on with Antiques & Collectibles categories.
"Understanding Single-Cell Omics: A Beginner's Guide to Single-Cell Omics Techniques - Exploring the Diversity of Life through Omics" provides an accessible introduction to the transformative field of single-cell omics. Delving into the intricacies of cellular analysis, this comprehensive guide navigates readers through the fundamental concepts and cutting-edge techniques that underpin single-cell omics research. From unraveling the complexities of gene expression patterns to deciphering cellular interactions within tissues, this book equips beginners with the essential knowledge and practical insights needed to embark on their journey into single-cell omics. Through clear explanations and real-world examples, readers will discover how single-cell omics is revolutionizing our understanding of biology and unraveling the mysteries of cellular diversity across organisms. Covering a range of topics including spatial transcriptomics, in situ sequencing, and single-cell proteomics, this guide illuminates the diverse applications of single-cell omics in fields such as developmental biology, cancer research, immunology, neuroscience, and regenerative medicine. Ethical considerations, data integration methods, and resources for further exploration are also discussed, providing readers with a holistic understanding of the field. Whether you're a student, researcher, or enthusiast, "Understanding Single-Cell Omics" serves as an invaluable companion for unlocking the potential of single-cell analysis and delving into the fascinating world of cellular heterogeneity and function. Get ready to embark on a journey of discovery and exploration as you unravel the intricacies of life at the single-cell level through the lens of omics technology.
Single Cell Omics
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Author : Debmalya Barh
language : en
Publisher: Academic Press
Release Date : 2019-07-31
Single Cell Omics written by Debmalya Barh and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-07-31 with Medical categories.
Single-cell Omics, Volume 2: Advances in Applications provides the latest single-cell omics applications in the field of biomedicine. The advent of omics technologies have enabled us to identify the differences between cell types and subpopulations at the level of the genome, proteome, transcriptome, epigenome, and in several other fields of omics. The book is divided into two sections: the first is dedicated to biomedical applications, such as cell diagnostics, non-invasive prenatal testing (NIPT), circulating tumor cells, breast cancer, gliomas, nervous systems and autoimmune disorders, and more. The second focuses on cell omics in plants, discussing micro algal and single cell omics, and more. This book is a valuable source for bioinformaticians, molecular diagnostic researchers, clinicians and several members of biomedical field interested in understanding more about single-cell omics and its potential for research and diagnosis.
Single Cell Analysis
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Author :
language : en
Publisher:
Release Date : 2010
Single Cell Analysis written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010 with categories.
Cellular heterogeneity arising from stochastic expression of genes, proteins, and metabolites is a fundamental principle of cell biology, but single cell analysis has been beyond the capabilities of 'Omics' technologies. This is rapidly changing with the recent examples of single cell genomics, transcriptomics, proteomics, and metabolomics. The rate of change is expected to accelerate owing to emerging technologies that range from micro/nanofluidics to microfabricated interfaces for mass spectrometry to third- and fourth-generation automated DNA sequencers. As described in this review, single cell analysis is the new frontier in Omics, and single cell Omics has the potential to transform systems biology through new discoveries derived from cellular heterogeneity.
Single Cell Omics Of Neuronal Cells
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Author : Jonathan V. Sweedler
language : en
Publisher: Springer Nature
Release Date : 2022-08-10
Single Cell Omics Of Neuronal Cells written by Jonathan V. Sweedler and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-08-10 with Medical categories.
This volume discusses the latest techniques used in the diverse fields of single cell ‘omics and covers topics such as quantifying the single cell transcriptome; isolation of cells in nanoliter volumes for single cell proteomics measurements by nano-LC-MS/MS; and single cell protein characterization by immunoblotting. A wide range of methodologies are highlighted, ranging from high-yield chemical amplification to mass spectrometry and nanotechnology for the analysis of the chemical constituents of cells. In the Neuromethods series style, chapters include both in-depth overviews, as well as detailed protocols that provide the key advice from specialists you need to get successful results in your laboratory. Cutting-edge and comprehensive, Single Cell ‘Omics of Neuronal Cells is a valuable resource for experienced and novice researchers interested in learning more about this field and its future developments.
Single Cell Omics
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Author : BCC Research
language : en
Publisher:
Release Date : 2017-12
Single Cell Omics written by BCC Research and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-12 with categories.
Single Cell Omics In Microbiome Research
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Author : Christian Rinke
language : en
Publisher: Springer Nature
Release Date : 2025-12-03
Single Cell Omics In Microbiome Research written by Christian Rinke and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2025-12-03 with Science categories.
This volume introduces the revolutionary field of microbial single-cell omics, a suite of cutting-edge techniques that uncover the genetic, functional, and metabolic signatures of individual microbial cells. From the discovery of new microbial lineages to decoding virus-host interactions, this book highlights the transformative impact of single-cell genomics, transcriptomics, proteomics, and metabolomics. With expert protocols and innovative workflows, the book provides practical guidance on fluorescence-activated cell sorting (FACS), microfluidic encapsulation, and advanced genome amplification techniques. It also explores targeted methods, like FISH-FACS for recovering rare microbes, activity-based cell separation, and multi-omics approaches that integrate genomic, transcriptomic, and proteomic data. The authors discuss future developments and the immense benefits of analysing and understanding microbes one cell at a time, offering a glimpse into how this knowledge can transform microbial ecology, biomedicine, and biotechnology. The book is aimed at researchers and students of environmental microbiology who wish to expand their knowledge of this ground-breaking approach and the underlying technology.
Single Cell Omics Analyses In Cardiovascular Diseases
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Author :
language : en
Publisher: Frontiers Media SA
Release Date : 2024-05-14
Single Cell Omics Analyses In Cardiovascular Diseases written by and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-05-14 with Medical categories.
Single-cell OMICs analyses have recently become one of the most promising tools to probe biology at the cellular level, in large part due to its ability to address issues beyond the bulk analysis – a window into cellular heterogeneity. The ability to profile transcriptomic, epigenomic, proteomics, and metabolomics at the single cell level including more recently the spatial information has enhanced our ability to understand interactions between biomolecules in different contexts leading to the discovery of specific cellular subpopulations as well as biological mechanisms underlying pathologies which may be amenable to therapeutic interventions. The scale and availability of a variety of technologies to measure intricate molecular details have provided an impetus to research in many disease areas, including cardiovascular medicine.