Light And Electron Microscopy
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Light And Electron Microscopy
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Author : Elizabeth M. Slayter
language : en
Publisher: Cambridge University Press
Release Date : 1992-10-30
Light And Electron Microscopy written by Elizabeth M. Slayter and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1992-10-30 with Science categories.
The compound optical microscope, in its various modern forms, is probably the most familiar of all laboratory instruments and the electron microscope, once an exotic rarity, has now become a standard tool in biological and materials research. Both instruments are often used effectively with little knowledge of the relevant theory, or even of how a particular type of microscope functions. Eventually however, proper use, interpretation of images and choices of specific applications demand an understanding of fundamental principles. This book describes the principles of operation of each type of microscope currently available and of use to biomedical and materials scientists. It explains the mechanisms of image formation, contrast and its enhancement, accounts for ultimate limits on the size of observable details (resolving power and resolution) and finally provides an account of Fourier optical theory. Principles behind the photographic methods used in microscopy are also described and there is some discussion of image processing methods. The book will appeal to graduate students and researchers in the biomedical sciences, and it will be helpful to students taking a course involving the principles of microscopy.
Bioimaging
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Author : Douglas E. Chandler
language : en
Publisher: Jones & Bartlett Publishers
Release Date : 2009
Bioimaging written by Douglas E. Chandler and has been published by Jones & Bartlett Publishers this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009 with Medical categories.
The Development Of Microscopy Revolutionized The World Of Cell And Molecular Biology As We Once Knew It And Will Continue To Play An Important Role In Future Discoveries. Bioimaging: Current Concepts In Light And Electron Microscopy Is The Optimal Text For Any Undergraduate Or Graduate Bioimaging Course, And Will Serve As An Important Reference Tool For The Research Scientist. This Unique Text Covers, In Great Depth, Both Light And Electron Microscopy, As Well As Other Structure And Imaging Techniques Like X-Ray Crystallography And Atomic Force Microscopy. Written In A User-Friendly Style And Covering A Broad Range Of Topics, Bioimaging Describes The State-Of-The-Art Technologies That Have Powered The Field To The Forefront Of Cellular And Molecular Biological Research.
An Introduction To Microscopy By Means Of Light Electrons X Rays Or Ultrasound
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Author : Eugene Rochow
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
An Introduction To Microscopy By Means Of Light Electrons X Rays Or Ultrasound written by Eugene Rochow and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Technology & Engineering categories.
Many people look upon a microscope as a mere instrument(l); to them microscopy is instrumentation. Other people consider a microscope to be simply an aid to the eye; to them microscopy is primarily an expan sion of macroscopy. In actuality, microscopy is both objective and sub jective; it is seeing through an instrument by means of the eye, and more importantly, the brain. The function of the brain is to interpret the eye's image in terms of the object's structure. Thought and experience are required to distinguish structure from artifact. It is said that Galileo (1564-1642) had his associates first look through his telescope microscope at very familiar objects to convince them that the image was a true representation of the object. Then he would have them proceed to hitherto unknown worlds too far or too small to be seen with the un aided eye. Since Galileo's time, light microscopes have been improved so much that performance is now very close to theoretical limits. Electron microscopes have been developed in the last four decades to exhibit thousands of times the resolving power of the light microscope. Through the news media everyone is made aware of the marvelous microscopical accomplishments in imagery. However, little or no hint is given as to what parts of the image are derived from the specimen itself and what parts are from the instrumentation, to say nothing of the changes made during preparation of the specimen.
Introduction To Microscopy By Means Of Light Electrons X Rays Or Acoustics
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Author : Theodore G. Rochow
language : en
Publisher: Springer Science & Business Media
Release Date : 1994-07-31
Introduction To Microscopy By Means Of Light Electrons X Rays Or Acoustics written by Theodore G. Rochow and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994-07-31 with Science categories.
Following three printings of the First Edition (1978), the publisher has asked for a Second Edition to bring the contents up to date. In doing so the authors aim to show how the newer microscopies are related to the older types with respect to theoretical resolving power (what you pay for) and resolution (what you get). The book is an introduction to students, technicians, technologists, and scientists in biology, medicine, science, and engineering. It should be useful in academic and industrial research, consulting, and forensics; how ever, the book is not intended to be encyclopedic. The authors are greatly indebted to the College of Textiles of North Carolina State University at Raleigh for support from the administration there for typing, word processing, stationery, mailing, drafting diagrams, and general assistance. We personally thank Joann Fish for word process ing, Teresa M. Langley and Grace Parnell for typing services, Mark Bowen for drawing graphs and diagrams, Chuck Gardner for photographic ser vices, Deepak Bhattavahalli for his work with the proofs, and all the other people who have given us their assistance. The authors wish to acknowledge the many valuable suggestions given by Eugene G. Rochow and the significant editorial contributions made by Elizabeth Cook Rochow.
Introduction To Electron Microscopy
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Author : Saul Wischnitzer
language : en
Publisher:
Release Date : 1962
Introduction To Electron Microscopy written by Saul Wischnitzer and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1962 with Electron microscopes categories.
Basic theory of electron microscopy; Nature of light beams; Resolution; Diffraction; Limit of resolution; Nature of electron beams; Electron emission; Electron optics; Introduction; Magnetic fields; Action of magnetic fields as lenses; Magnetic focusing; Evolution of magnetic lenses for electron microscopy; Analogy between light and electron microscopes; The electron microscope; Illuminating system; Electron gun; Filament; Shield; Anode; Non-biased and biased guns; Self-biased gun; Operation of self-biased gun; Condenser lens; Aperture angle; Intensity; Depth of field; Condenser lens operation; Imaging system; Objective lens; Pole pieces; Lens aberration spherical and chromatic; Limitation of objective lens aberration; Contrast and image formation; Objective lens operation; Projecton lens; magnification and final image formation; Range of magnification; Image translating system; Fluorescent observation screen; Photographic recording; Summary of general considerations in image translation; Beam intensity level; Choice of photographic emulsion; Other electron microscope components; Specimen chamber and holder; Photographic chamber; Vacuum supply; Filament current supply; High voltage supply; Operational requirements; Alignment; Electron gun-condenser alignment; Lens alignment; Detection of lens asymmetry; Disturbances; Magnetic fields; Mechanical; Specimen instability; Contamination; Vacuum leaks; Operation of the electron microscope; Manipulation; Photography; Determination of magnification; Test of resolution; Differences between light and electron microscope.
Correlative Light And Electron Microscopy
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Author : Thomas Muller-Reichert
language : en
Publisher: Academic Press
Release Date : 2012-08-10
Correlative Light And Electron Microscopy written by Thomas Muller-Reichert and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-08-10 with Science categories.
The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM. Covers many areas of CLEM by the best international scientists in the field Includes a brief history and overview on CLEM methods
Electron Microscopy And Analysis
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Author : Peter J. Goodhew
language : en
Publisher: CRC Press
Release Date : 1988-04-25
Electron Microscopy And Analysis written by Peter J. Goodhew and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1988-04-25 with Science categories.
A comprehensive introductory text, extensively revised and updated to cover the physical basis and operation of the common types of electron microscope with illustrations of their applications. In addition, electron microscopy is compared with other modern techniques for examining both crystalline and non-crystalline materials.
Understanding Light Microscopy
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Author : Jeremy Sanderson
language : en
Publisher: John Wiley & Sons
Release Date : 2019-03-04
Understanding Light Microscopy written by Jeremy Sanderson and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-03-04 with Science categories.
Introduces readers to the enlightening world of the modern light microscope There have been rapid advances in science and technology over the last decade, and the light microscope, together with the information that it gives about the image, has changed too. Yet the fundamental principles of setting up and using a microscope rests upon unchanging physical principles that have been understood for years. This informative, practical, full-colour guide fills the gap between specialised edited texts on detailed research topics, and introductory books, which concentrate on an optical approach to the light microscope. It also provides comprehensive coverage of confocal microscopy, which has revolutionised light microscopy over the last few decades. Written to help the reader understand, set up, and use the often very expensive and complex modern research light microscope properly, Understanding Light Microscopy keeps mathematical formulae to a minimum—containing and explaining them within boxes in the text. Chapters provide in-depth coverage of basic microscope optics and design; ergonomics; illumination; diffraction and image formation; reflected-light, polarised-light, and fluorescence microscopy; deconvolution; TIRF microscopy; FRAP & FRET; super-resolution techniques; biological and materials specimen preparation; and more. Gives a didactic introduction to the light microscope Encourages readers to use advanced fluorescence and confocal microscopes within a research institute or core microscopy facility Features full-colour illustrations and workable practical protocols Understanding Light Microscopy is intended for any scientist who wishes to understand and use a modern light microscope. It is also ideal as supporting material for a formal taught course, or for individual students to learn the key aspects of light microscopy through their own study.
Methods Of Preparation For Electron Microscopy
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Author : David G. Robinson
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Methods Of Preparation For Electron Microscopy written by David G. Robinson and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
In 1939, when the electron optics laboratory of Siemens & Halske Inc. began to manufacture the first electron microscopes, the biological and medical profes sions had an unexpected instrument at their disposal which exceeded the reso lution of the light microscope by more than a hundredfold. The immediate and broad application of this new tool was complicated by the overwhelming prob lems inherent in specimen preparation for the investigation of cellular struc tures. The microtechniques applied in light microscopy were no longer appli cable, since even the thinnest paraffin layers could not be penetrated by electrons. Many competent biological and medical research workers expressed their anxiety that objects in high vacuum would be modified due to complete dehydration and the absorbed electron energy would eventually cause degrada tion to rudimentary carbon backbones. It also seemed questionable as to whether it would be possible to prepare thin sections of approximately 0. 5 11m from heterogeneous biological specimens. Thus one was suddenly in posses sion of a completely unique instrument which, when compared with the light microscope, allowed a 10-100-fold higher resolution, yet a suitable preparation methodology was lacking. This sceptical attitude towards the application of electron microscopy in bi ology and medicine was supported simultaneously by the general opinion of colloid chemists, who postulated that in the submicroscopic region of living structures no stable building blocks existed which could be revealed with this apparatus.
Biological Electron Microscopy
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Author : Michael J. Dykstra
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Biological Electron Microscopy written by Michael J. Dykstra and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
In this practical text, the author covers the fundamentals of biological electron microscopy - including fixation, instrumentation, and darkroom work - to provide an excellent introduction to the subject for the advanced undergraduate or graduate student.