Immunocytochemical Methods And Protocols

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Immunocytochemical Methods And Protocols

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Author : Constance Oliver
language : en
Publisher: Humana Press
Release Date : 2012-04-06

Immunocytochemical Methods And Protocols written by Constance Oliver and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-04-06 with Medical categories.

Antibodies tagged with fuorescent markers have been used in histochemistry for over 50 years. Although early applications were focused on the detection of microbial antigens in tissues, the use of immunocytochemical methods now has spread to include the det- tion of a wide array of antigens including proteins, carbohydrates, and lipids from virtually any organism. Today, immunohistochemistry is widely used to identify, in situ, various components of cells and tissues in both normal and pathological conditions. The method gains its strength from the extremely sensitive interaction of a specifc antibody with its antigen. For some scientifc areas, books have been published on applications of immu- cytochemical techniques specifc to that area. What distinguished Immunocytochemical Methods and Protocols from earlier books when it was frst published was its broad appeal to investigators across all disciplines, including those in both research and clinical settings. The methods and protocols p- sented in the frst edition were designed to be general in their application; the accompa- ing “Notes” provided the reader with invaluable assistance in adapting or troubleshooting the protocols. These strengths continued to hold true for the second edition and again for the third edition. Since the publication of the frst edition, the application of immuno- tochemical techniques in the clinical laboratory has continued to rise and this third edition provides methods that are applicable to basic research as well as to the clinical laboratory.

Immunohistochemistry And Immunocytochemistry

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Author : Luis Del Valle
language : en
Publisher: Humana
Release Date : 2022-12-18

Immunohistochemistry And Immunocytochemistry written by Luis Del Valle and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-12-18 with Medical categories.

This volume provides a comprehensive reference guide for researchers to study the applications of labeled antibodies. Chapters guide reader through the theory and practice of immunohistochemistry, immunocytochemistry and immunofluorescence techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and useful tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Immunohistochemistry and Immunofluorescence: Methods and Protocols aims to be a useful practical guide to scientists to help further their study in this field

Methods In Molecular Biology Immunocytochemical Methods And Protocols

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Author : John M. Walker
language : en
Publisher:
Release Date : 1984

Methods In Molecular Biology Immunocytochemical Methods And Protocols written by John M. Walker and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1984 with Molecular biology -- v.236. Plant functional genomics -- v.237. G protein signaling -- v.238. Biopolymer methods in tissue engineering -- v.239. Cell migration in inflammation and immunity -- v.240. Mammalian artificial chromosomes -- v.241. Cell cycle checkpoint control protocols -- v.242. Atomic force microscopy -- v.243. Chiral separations: Methods and protocols -- v.244. Protein purification protocols -- v.245-6. Gene delivery to mammalian cells -- v.247. Drosophila cytogenetics protocols -- v.248. Antibody engineering -- v.249. Cytokine protocols -- v.251. HPLC of peptides and proteins: Methods and protocols -- v.265. RNA interference, editing, and modification -- v.274. Photosynthesis research protocols -- v.318. Plant cell culture protocols -- v.323. Arabidopsis protocols categories.

Biochemicals And Reagents

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Author :
language : en
Publisher:
Release Date :

Biochemicals And Reagents written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on with categories.

Protocols For Gene Transfer In Neuroscience

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Author : P. R. Lowenstein
language : en
Publisher:
Release Date : 1996-07-25

Protocols For Gene Transfer In Neuroscience written by P. R. Lowenstein and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996-07-25 with Medical categories.

Protocols for Gene Transfer in Neuroscience Towards Gene Therapy of Neurological Disorders Edited by P. R. Lowenstein University of Manchester, UK and L. W. Enquist Princeton University, USA The brain and the cells that compose it have always presented unique barriers to any kind of outside interventions, including drug delivery, surgical procedures and the genetic manipulation of cells. However, these barriers are now being bypassed by the new technology of gene delivery. Protocols for Gene Transfer in Neuroscience is the first practical guide to gene delivery, providing over 150 easy to follow, step-by-step protocols, together with troubleshooting hints and the theoretical background needed for neuroscientists to understand, adapt and expand the technology as needed. Written by a team of international expert neuroscientists, geneticists, molecular biologists and biochemists, Protocols for Gene Transfer in Neuroscience describes the latest techniques from the top gene-delivery labs, ranging from physical transfection techniques, through the use of a wide range of viral vectors, to retrovirally transduced cell and bone marrow transplantation. Several variations of a single protocol are given, thus demonstrating how different labs have solved a common problem in different ways. The protocols are also indexed and cross-referenced so that an investigator can find all the details required for a particular problem.

Cytochemical Methods In Neuroanatomy

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Author : Victoria Chan-Palay
language : en
Publisher:
Release Date : 1982

Cytochemical Methods In Neuroanatomy written by Victoria Chan-Palay and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1982 with Medical categories.

Immunocytochemical Technology

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Author : Gwen V. Childs
language : en
Publisher:
Release Date : 1986

Immunocytochemical Technology written by Gwen V. Childs and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1986 with Science categories.

Neurochemistry

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Author : Anthony John Turner
language : en
Publisher: Oxford University Press, USA
Release Date : 1987

Neurochemistry written by Anthony John Turner and has been published by Oxford University Press, USA this book supported file pdf, txt, epub, kindle and other format this book has been release on 1987 with Medical categories.

Neurobiology has undergone a revolution with many advances dependent on new techniques and approaches. This fully updated second edition reflects the recent growth areas in this subject. Its major themes are the fractionation and study of cellular and subcellular components of the nervoussystem, chemical neuroanatomy, and the measurement of neuronal metabolism in vivo and in vitro. Topics include the neuronal cytoskeleton; isolation of synaptosomes and their subcellular components; aspects of mitochondrial defects in neurones; and synaptic transport processes exemplified by the GABAtransporter. This is an invaluable practical laboratory manual for all researchers in neurobiology; it contains step-by-step protocols and a troubleshooting guide, as well as examples of experimental data and their interpretation.

Handbook Of Chemical Neuroanatomy Analysis Of Neuronal Microcircuits And Synaptic Interactions

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Author : Anders Björklund
language : en
Publisher:
Release Date : 1983

Handbook Of Chemical Neuroanatomy Analysis Of Neuronal Microcircuits And Synaptic Interactions written by Anders Björklund and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1983 with Histochemistry categories.

Atlas Of Diagnostic Cytopathology

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Author : Barbara F. Atkinson
language : en
Publisher: Saunders
Release Date : 2004

Atlas Of Diagnostic Cytopathology written by Barbara F. Atkinson and has been published by Saunders this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Medical categories.

The New Edition of the field's standard sourcebook delivers thoroughly revised and up-to-date coverage of aspiration and exfoliative cytology and its impact on the practice of anatomic pathology today. Over 1,700 all-new, full-color illustrations capture the appearance of a complete range of cytopathology specimens, their histologic correlations, and the results of relevant ancillary studies. Each chapter discusses the challenges of that specimen site; describes the diagnostic criteria and differential diagnosis; and explains how the diagnosis is reached. Presents comprehensive, pictorial coverage of the cytopathology of every organ system. Includes diagnostic rules and tips. Discusses medical liability and the practice of cytopathology. Highlights key information in tables and boxes. Reflects all of the changes in both aspiration and exfoliative cytology, and discusses the utility of different cytologic stains in detail. Features an expanded section on gynecologic cytopathology that includes the diagnostic criteria for breast cancer and the associated diagnostic applications. Discusses new pathologic entities and the Betheseda System of Nomenclature for interpreting Pap tests. Covers ancillary techniques of diagnostic cytopathology Addresses ethical and medicological issues in new chapters. Offers over 1,700 all-new, full-color illustrations that clearly demonstrate procedures as well as more Diff Quik stains for fine needle aspirates. Includes true histologic correlations and discusses the role of all ancillary tests.