Drosophila Protocols

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Drosophila Protocols

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Author : William Sullivan
language : en
Publisher: CSHL Press
Release Date : 2000

Drosophila Protocols written by William Sullivan and has been published by CSHL Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000 with Medical categories.

This exceptional laboratory manual describes thirty-seven procedures most likely to be used in the next decade for molecular, biochemical, and cellular studies on Drosophila. They were selected after extensive consultation with the research community and rigorously edited for clarity, uniformity, and conciseness.The methods included permit investigation of chromosomes, cell biology, molecular biology, genomes, biochemistry, and development. Each protocol includes the basic information needed by novices, with sufficient detail to be valuable to experienced investigators. Each method is carefully introduced and illustrated with figures, tables, illustrations, and examples of the data obtainable. The book's appendices include key aspects of Drosophila biology, essential solutions, buffers, and recipes.An evolution of Michael Ashburner's 1989 classic Drosophila: A Laboratory Manual, this book is an essential addition to the personal library of Drosophila investigators and an incomparable resource for other research groups with goals likely to require fly-based technical approaches.

Drosophila

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Author : Christian Dahmann
language : en
Publisher: Springer Nature
Release Date : 2022-08-18

Drosophila written by Christian Dahmann and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-08-18 with Science categories.

This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and cell lineage tracing, protein inhibition and tagging, optogenetic and optochemical control of tissue mechanics, AFM measurements, sample preparation for electron microscopy, live imaging of different tissues and organs, and quantitative image analysis. Written in the format of the highly successful Methods in Molecular Biology series, chapters include an introduction to their topic, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls Authoritative and cutting-edge, Drosophila: Methods and Protocols, Third Edition serves as a useful and practical guide to new researchers and experts using Drosophila as a model system. Chapter 7 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Drosophila Cytogenetics Protocols

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Author : Daryl S. Henderson
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03

Drosophila Cytogenetics Protocols written by Daryl S. Henderson and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.

Leading drosophilists describe in step-by-step detail all the essential techniques for studying Drosophila chromosomes and suggest new avenues for scientific exploration. The chapters emphasize specimen preparation (from dissection to mounting) and cover both polytene and mitotic/meiotic chromosomes in depth. Each fully tested and readily reproducible protocol offers a background introduction, equipment and reagent lists, and tips on troubleshooting and avoiding pitfalls. A cutting-edge FISH and immunolocalization technique will be important for discovering how DNA sequence influences higher-order chromosome architecture and ultimately gene expression.

Drosophila Protocols

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Author : William Sullivan
language : en
Publisher:
Release Date : 2000

Drosophila Protocols written by William Sullivan and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000 with Drosophila categories.

Short Protocols In Molecular Biology

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Author : Frederick M. Ausubel
language : en
Publisher: Current Protocols
Release Date : 2002-11-05

Short Protocols In Molecular Biology written by Frederick M. Ausubel and has been published by Current Protocols this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-11-05 with Medical categories.

Expanded to 2 volumes, Short Protocols in Molecular Biology, Fifth Edition, provides condensed descriptions of more than 700 methods compiled from Current Protocols in Molecular Biology. Includes new chapters on chromatin assembly and analysis, nucleic acid arrays, generation and use of combinatorial libraries, discovery and analysis of differentially expressed genes in single cells and cell populations. The book is specifically designed to provide quick access to step-by-step instructions for the essential methods used in every major area of molecular biological research Short Protocols in Molecular Biology, Fifth Edition is an authoritative and indispensable guide for all life scientists, researchers, and students at the graduate and advanced undergraduate level.

Tissue In Situ Hybridization

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Author : Trevor Jowett
language : en
Publisher: Wiley-Interscience
Release Date : 1997-02-05

Tissue In Situ Hybridization written by Trevor Jowett and has been published by Wiley-Interscience this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997-02-05 with Medical categories.

TISSUE IN SITU HYBRIDIZATION Methods in Animal Development Trevor Jowett The European Molecular Biology Organization (EMBO) course on tissue in situ hybridization in animal developmental biology has served as an important and highly respected forum for the latest advances in the methodology of this valuable research tool. Developed from EMBO course materials, Tissue In Situ Hybridization provides scientists and researchers worldwide with detailed coverage of new approaches, techniques, and protocols, along with up-to-date information on more established procedures. Focusing particularly on the two-color in situ hybridization method to whole-mount embryos and tissue sections, this practical resource also compares different methods of producing differentially colored signals, and includes the results of protocol experiments with fluorescent and other alternative in situ hybridization techniques. Special features include: Photographic examples—including color plates—to complement the text Clear explanations of the principles underlying different methods Detailed discussion and comparison of the different methods Valuable troubleshooting advice and practical guidance Comprehensive index, allowing quick and easy access to specific topics Compiled by a leading expert in the field, Tissue In Situ Hybridization is an indispensable asset to professionals and researchers working in the areas of developmental, cell, and molecular biology.

Epigenetics

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Author : Bruce Stillman
language : en
Publisher: CSHL Press
Release Date : 2004

Epigenetics written by Bruce Stillman and has been published by CSHL Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Science categories.

The importance of epigenetic regulation of gene expression, particularly in higher organisms, is now clear and the 2004 Cold Spring Harbor Annual Symposium provided many new examples as well as insights into the underlying mechanisms. The resulting volume—with over 60 papers from experts across the field—covers various aspects of nuclear organization and dynamics; genomic imprinting, chromosomal inactivation, and other examples of gene silencing; the histone and DNA modifications associated with these conditions; and the roles of RNA and protein regulators in establishing and maintaining these states.

Genome Research

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Author :
language : en
Publisher:
Release Date : 2006

Genome Research written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006 with Genetics categories.

Drosophila Oogenesis

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Author : Michelle S. Giedt
language : en
Publisher: Springer Nature
Release Date : 2023-01-30

Drosophila Oogenesis written by Michelle S. Giedt and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-01-30 with Science categories.

This detailed volume compiles numerous methods to explore fly oogenesis. Beginning with updated protocols from isolating and staining the ovary to numerous imaging techniques and genetic protocols for cell-specific assessment and CRISPR-mediated mutagenesis, the volume continues by detailing techniques from assessing cytoskeletal structures to uncovering protein-protein interactions, with closing chapters exploring how Drosophila oogenesis can be used in the classroom and in outreach programs to increase interest in biomedical research, STEM education, and STEM careers. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Drosophila Oogenesis: Methods and Protocols serves as an ideal guide for both new and expert researchers working with this robust and versatile model organism.

Emerging Model Organisms

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Author :
language : en
Publisher:
Release Date : 2009

Emerging Model Organisms written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009 with Biological models categories.

"Until recently, a small number of model organisms has been the focus of most research in molecular, cellular, and developmental biology. But in the last few years, due in part to increased interest in questions of evolution, technical advances in selectively altering gene expression patterns, and reduced costs of genome sequencing, the range of organisms used for research is greatly expanding. Emerging Model Organisms, Volume 1, introduces the reader to this new generation of model organisms, providing a diverse catalog of potential species useful for extending research in new directions. In this volume, leading experts provide chapters on 23 emerging model systems, ranging from bat and butterfly to cave fish and choanoflagellates; cricket and finch to quail, snail, and tomato. Subsequent releases of the Emerging Model Orgaisms series, already in preparation, will focus on additional species. Richard Behringer, Rob Krumlauf, Sandy Johnson, Mike Levine, Nipam Patel, Neelima Sinha." -- Publisher.