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Advanced Methods In Protein Sequence Determination


Advanced Methods In Protein Sequence Determination
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Molecular Biology Biochemistry And Biophysics


Molecular Biology Biochemistry And Biophysics
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Author :
language : en
Publisher:
Release Date : 1986

Molecular Biology Biochemistry And Biophysics written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1986 with Biochemistry categories.




Advanced Methods In Protein Sequence Determination


Advanced Methods In Protein Sequence Determination
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Author : Saul B. Needleman
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06

Advanced Methods In Protein Sequence Determination written by Saul B. Needleman and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.


Confusion now hath made his masterpiece Macbeth II iii 72 Whence and what are those execrable shape? Paradise Lost Ib 1 681 Confusion worse confounded Paradise Lost Ib 1 995 When the manuscript for the first part of this book was proposed, it was anticipated that the discussion of the entire field of protein se quencing could be covered in a single volume - from purification and characterization of the protein through fragmentation by chemical or enzymic means and, finally, to reassembly of the identified individual peptides into the reconstructed total sequence. It soon became evident that this would not be possible. While the intent was to restrict the expose of procedures only to that information concerned with "hands on" wet chemistry, it became apparent that a thorough presentation would require, in addition, a discussion of certain instrumental and more theoretical approaches not included in the first volume. Furthermore, the entire understanding of the field of protein sequencing has advanced appreciably since the inception of this book. The purpose of the first volume was to provide practical information in sufficient detail to permit the researcher to undertake the actual sequencing procedures in his own laboratory.



Methods In Protein Sequence Analysis


Methods In Protein Sequence Analysis
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Author : K. Imahori
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-06-29

Methods In Protein Sequence Analysis written by K. Imahori and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-06-29 with Science categories.


The Ninth International Conference on Methods in Protein Sequence Analysis was held for the first time in Asia from September 20 to September 24, 1992 in Otsu (a city near Kyoto), Japan. Approximately 400 delegates attended the meeting. Forty papers were presented orally and 147 poster presentations were discussed. Academic sessions were held from early in the morning until late in the evening. We are confident that the Conference was successful in providing up-to-date information about methods in protein sequence analysis to all participants. Moreover, with the knowledge and understanding of the present standard of various methods of analysis that are being used and will be used, we were able to clarify areas that need to be evaluated, to be improved and be explored further. Major topics in the Conference mostly covered areas in the methodology of protein sequence analysis, such as: micropreparation and microsequencing of proteins, mass spectrometry, post-translational modification, prediction and database analysis, and analysis of protein structures of special interests. The evolution of genetic engineering in molecular biology has greatly accelerated the accumulation of knowledge on the amino acid sequence of novel proteins regardless of whether they are expressed or not expressed in living organisms. In the early stage of accumulation of structural information, the amino acid sequence itself is worthy of notice.



Methods In Protein Sequence Analysis


Methods In Protein Sequence Analysis
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Author : Brigitte Wittmann-Liebold
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06

Methods In Protein Sequence Analysis written by Brigitte Wittmann-Liebold and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.


"Methods in Protein Sequence Analysis - 1988" - contains selected contributions on modern protein- analytical techniques as presented by speakers at the Seventh International Conference on "Methods in Protein Sequence Analysis", held from July 3rd to July 8th, 1988 in Berlin. The book contains information on new methodologies for sensitive amino acid analysis, N- and C-terminal sequence analysis, and protein and peptide purification. In addition recent mass spectrometric approaches are described, as an alter native technique to the common stepwise degradative sequence analysis of polypeptides by the Edman method. The book presents new possibilities in the design of sequencers and sophisticated equipment for the structural analysis of peptides and proteins. It describes practical approaches for the investigation of protein domains and protein complexes, and contains review chapters on the crystallization of cell organelles as well as on recent theoretical aspects of protein folding mechanisms. The nature of protein folding is not yet understood, but further advances in this area would greatly enhance our present knowledge of protein structure and function. Further, the book gives examples of the application of gene technology to protein characterization and to the design of new proteins. This enables new studies on the structure and function of proteins to be made, and opens up efficient approaches to the design of drugs.



Protein Peptide Sequence Analysis Current Methodologies


Protein Peptide Sequence Analysis Current Methodologies
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Author : A.S. Bhown
language : en
Publisher: CRC Press
Release Date : 2018-02-01

Protein Peptide Sequence Analysis Current Methodologies written by A.S. Bhown and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-02-01 with Medical categories.


This book is an attempt to provide in a single source current state-of-the-art methodologies for protein sequence analysis. It is hoped that these various chapters are presented in such a way that both the newcomer and the established protein chemist will find useful information and directions to new techniques. This book offers a rich array of techniques and methods for sequencing proteins and peptides. It should meet the expectations of investigators in protein chemistry who wish to update their knowledge of sequencing techniques, and of those who wish to reacquaint themselves with the best available current technologies.



Advanced Methods In Protein Microsequence Analysis


Advanced Methods In Protein Microsequence Analysis
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Author : Brigitte Wittmann-Liebold
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06

Advanced Methods In Protein Microsequence Analysis written by Brigitte Wittmann-Liebold and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.


Much of the recent spectacular progress in the biological sciences can be at tributed ot the ability to isolate, analyze, and structurally characterize proteins and peptides which are present in cells and cellular organelles in only very small amounts. Recent advances in protein chemistry and in particular the application of new micromethods have led to fruitful advances in the understanding of basic cellular processes. Areas where protein-chemical studies have resulted in interest ing discoveries include the peptide hormones and their release factors, growth factors and oncogenes, bioenergetics, proton pumps and ion pumps and chan nels, topogenesis and protein secretion, molecular virology and immunology, membrane protein analysis, and receptor research. In fact, the key methods are now on hand to unravel many of the major outstanding problems of molecular biology and in particular questions of fundamental interest which relate to devel opmental biology and specificity in cell-cell interaction. In this volume we have assembled descriptions of procedures which have re cently been shown to be efficaceous for the isolation, purification, and chemical characterization of proteins and peptides that are only available in minute amounts. Emphasis is placed on well-established micromethods which have been tested and found useful in many laboratories by experienced investigators. The chapters are written by specialists, and describe a range of sensitive techniques which can be used by researchers working in laboratories with only modest resources and equipment.



Techniques In Molecular Biology


Techniques In Molecular Biology
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Author : J.M. Walker
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06

Techniques In Molecular Biology written by J.M. Walker and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.


The last few years have seen the rapid development of new methodology in the field of molecular biology. New techniques have been regularly introduced and the sensitivity of older techniques greatly improved upon. Developments in the field of genetic engineering in particular have contributed a wide range of new techniques. The purpose of this book therefore is to introduce the reader to a selection of the more advanced analytical and preparative techniques which the editors consider to be frequently used by research workers in the field of molecular biology. In choosing techniques for this book we have obviously had to be selective, and for the sake of brevity a knowledge of certain basic biochemical techniques and terminology has been assumed. However, since many areas of molecular biology are developing at a formidable rate and constantly generating new terminology, a glossary of terms has been included. The techniques chosen for this book are essentially based on those used in a series of workshops on 'techniques in molecular biology' that have been held at The Hatfield Polytechnic in recent years. In choosing these chapters we have taken into account many useful suggestions and observations made by participants at these workshops. Each chapter aims to describe both the theory and relevant practical details for a given technique, and to identify both the potential and limitations of the technique. Each chapter is written by authors who regularly use the technique in their own laboratories.



Concepts In Protein Engineering And Design


Concepts In Protein Engineering And Design
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Author : Paul Wrede
language : en
Publisher: Walter de Gruyter GmbH & Co KG
Release Date : 2020-10-12

Concepts In Protein Engineering And Design written by Paul Wrede and has been published by Walter de Gruyter GmbH & Co KG this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-10-12 with Science categories.


No detailed description available for "Concepts in Protein Engineering and Design".



High Performance Liquid Chromatography Of Peptides And Proteins


High Performance Liquid Chromatography Of Peptides And Proteins
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Author : Colin T. Mant
language : en
Publisher: CRC Press
Release Date : 2017-11-22

High Performance Liquid Chromatography Of Peptides And Proteins written by Colin T. Mant and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-11-22 with Medical categories.


This book consists of a series of 82 precise, easy-to-read articles by internationally renowned scientists and emphasizes the practical approach to HPLC with minimal theory, although the underlying principles for peptide and protein separations are clearly expressed. All of the major modes of microbore, ultrafast and analytical HPLC are discussed, including size-exclusion, ion-exchange, reversed-phase, hydrophobic interaction, and affinity and immunoaffinity chromatography. A section on preparative HPLC, including displacement techniques, is also presented. Problem-solving approaches to the separation of various classes of biologically active peptides and proteins are thoroughly explored, while the importance of peptide standards for monitoring column performance and for optimizing separation conditions is emphasized. Several articles focus on the choice of the correct detection method (electrochemical, UV, fluorescence), as well as the need for a proper knowledge of approaches to column and instrument maintenance and trouble-shooting. A section on predictive approaches deals with both computer simulation of peptide separations and peptide structure. The book also includes complementary techniques to HPLC, as well as other useful applications of HPLC. It enables both novice and experienced chromatographers to realize the full potential of this extremely powerful technique, in the process making an important contribution to scientific literature.



Memoirs Of The Institute For Protein Research Osaka University


Memoirs Of The Institute For Protein Research Osaka University
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Author : Ōsaka Daigaku. Tanpakushitsu Kenkyūjo
language : en
Publisher:
Release Date : 1983

Memoirs Of The Institute For Protein Research Osaka University written by Ōsaka Daigaku. Tanpakushitsu Kenkyūjo and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1983 with Proteins categories.